Monitoring Enzyme Catalysis using the VERTEX 80 FT-IR Spectrometer in Rapid Scan Mode

This application note describes the monitoring of the peptide product of the chymotrypsin-catalysed hydrolysis of N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide (Suc-AAPF-pNA) by FT-IR spectroscopy. This reaction can be followed by FT-IR due to the formation of a new C-terminal carboxylate group. The VERTEX 80 FT-IR spectrometer was shown to collect more than 100 spectra at resolution 16 cm-1 per second at the fastest mirror velocity (320 kHz).