Monitoring Neurite Morphology and Synapse Formation in Primary Neurons for Neurotoxicity Assessments and Drug Screening

This application note introduces a new method for measuring synaptic function utilizing the power of automated, quantitative, high-content cell-based imaging and analysis. The Thermo Scientific Synaptogenesis HCS Assay Reagents combined with the Thermo Scientific ArrayScan High Content Screening (HCS) Reader and Neuronal Profiling BioApplication enabled the automated quantitation of neuronal morphology and synapses in vitro. The Multiparameter Synaptogenesis Assay simultaneously identified and quantified neurites, pre- and post-synaptic structures and synapses in an automated manner.