Morphologic and Location-Based Classification of Differentiating Erythroid Lineage Cells using the ImageStream

In this application note human CD34-positive early hematopoietic cells were cultured to promote differentiation into erythroid lineage cells. During the differentiation program cells were sampled at 10, 13, or 16 days followed by staining with a nuclear dye and for expression of CD71 and glycophorin A, then analyzed immunophenotypically and morphologically on the ImageStream.