Multi-analyte, Automated, Microfluidic Immunoassay Platform

Protein analytes or biomarkers have traditionally been measured individually in ELISAs, which can attain a high degree of analytical specificity by testing only a single analyte with a dedicated antibody pair. However, the clinical field is becoming increasingly aware that multiple markers are associated with complex, multimodal, multivariate diseases including sepsis, rheumatoid arthritis, cancer, neurodegenerative diseases and traumatic brain injury. The application note demonstrate the measurement of protein analytes by Simple Plex™, a novel automated immunoassay platform that enabled simultaneous multi-analyte quantification, while retaining the sensitivity and specificity of single analyte ELISAs without the associated negatives of multi-analyte analysis of high sample volume requirements, slow reaction rates, high labor requirements, and cost inefficiencies when analyzing multiple analyte.