Multi-attribute quantification of LNP-mRNA therapeutics by FFF-MALS and DLS

Explore how multi-angle light scattering (MALS) with field-flow fractionation (FFF) can be used for high-precision characterization of lipid nanoparticle (LNP)-mRNA therapeutics. Unlike traditional dye-binding assays, this automatable method quantifies LNP size, concentration, and mRNA payload distribution. Applied to two COVID-19 vaccines, it reveals key differences in particle distribution and drug substance loading, ensuring quality control and regulatory compliance.