Multi-charged ion analysis of intact antibodies using a Q-TOF mass spectrometer

Explore the use of mass spectrometry for characterizing antibody drugs, including monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs). Shimadzu examines the challenges of structural heterogeneity in antibody production and introduces examples of analyzing pseudo ADCs with MALDI-TOF MS and trastuzumab with its LCMS-9030 quadrupole time-of-flight (Q-TOF) mass spectrometer. The LCMS-9030 integrates quadrupole technology with TOF capabilities for accurate mass measurement. Techniques like charge deconvolution aid in molecular weight and glycosylation analysis, ensuring drug quality and efficacy.