Multi-detector GPC/SEC analysis of polysaccharides using OMNISEC

Gel permeation chromatography (GPC) or, equivalently, size-exclusion chromatography (SEC), is a widely used technique to characterize a range of macromolecules, from bulk manufactured materials to natural polymers and proteins. This application note describes the analysis of three polysaccharide samples using advanced detection GPC/SEC, using Malvern’s OMNISEC Tetra Detection GPC/SEC System. The article highlights the benefits of using advanced GPC/SEC over conventional calibration, including the ability to measure absolute molecular weight, independent of molecular size and retention volume.