Multi-Parametric Assessment of Cell Phenotypes

Automated cellular imaging is an efficient method for analyzing compound effects on cell phenotypes including morphology, viability, and marker expression. Here, we demonstrate how the ImageXpress^® Pico Automated Cell Imaging System and CellReporterXpress Automated Imaging and Analysis Software is applied for phenotypic analysis of compound effects. Imaging and analysis methods provide tools for characterization of multiple readouts including the assessment of cell viability, characterization of cell shape, cell adhesion and spreading, cytoskeleton integrity, and mitochondria potential.