Multi-Parametric Electrophysiological Imaging of the Mammalian Heart In-Vivo using Photometrics’ Evolve 128 EMCCD Camera

Cardiac arrhythmia is one of the most common diseases encountered in clinical cardiology. High-speed electrophysiological imaging using fluorescent probes has yielded tremendous insights into the basic mechanisms of arrhythmias and the effects of anti-arrhythmic drugs. Two key parameters of interest are the cardiac action potential (AP) and intracellular calcium transient (CaT), which requires the use of red-shifted dyes to image in vivo. This case study demonstrates the first ever multi-parametric imaging of a mammalian heart in-vivo through whole blood.