Multiparameter Analysis of Murine Regulatory T-Cells and Dendritic Cells with the Attune NxT Flow Cytometer

Immunophenotyping is the analysis of heterogeneous populations of cells within mouse blood, splenocytes, and thymocytes for the purpose of identifying the presence and proportions of various cell populations. Flow cytometry is the method of choice for identifying cells within complex populations, as it allows for multiparameter analysis of thousands to millions of cells in a short time. This application note describes the use of the Attune NxT Flow Cytometer for 4-laser and 10-color immunophenotyping analysis of stained mouse splenocytes using a fixation and permeabilization protocol.