Multiparametric Analysis of Cytotoxicity using Cell Imaging in Combination with Luminescence

Cytotoxicity can be caused by different types of agents, including drugs, pathogens, immune cells and external stress factors such as heat. Particularly during the drug development process, where cytotoxicity remains one of the major causes of drug withdrawal, there is an urgent need for reliable and time-saving assay workflows that gain a more complete picture of the effects of cytotoxicity. This application demonstrates how multiparametric analysis of cytotoxicity, using different technologies, can be easily performed on the EnSight™ Multimode Plate Reader and how brightfield confluency analysis serves as a robust indicator for toxicity in adherent cells and can be easily applied in labeled or label-free cell-based assay.