Multiple focused CRISPR libraries from a single oligo pool

Functional genetic screening using the CRISPR/Cas9 system is a powerful tool for interrogating gene function at scale, but access to and implementation of this technology is constrained by existing pre-defined CRISPR sgRNA libraries.

In this application note, a single Twist Oligo Pool was used in combination with Custom Library Multiplexed Cloning, an easy-to-use bioinformatics and wet-lab workflow, to generate numerous high-quality, custom sgRNA libraries.