Multiplex Pluripotency Analysis of STEMCCA™ Vector-Reprogrammed iPS Cells using MILLIPLEX® MAP Magnetic Bead Assays

For the application of induced pluripotent stem cells (iPSC) and human embryonic stem cells (hESC) in research, it is crucial to characterize the identity and potency status of the pluripotent stem cells by measuring molecular markers during cultivation. This application note describes how MILLIPLEX® MAP multiplexed bead-based immunoassays, which use Luminex® xMAP® technology to provide a complementary assay to flow cytometry, can be used for quickly assessing the potency status of a cell population.