Multiplexed Assay for IL-6 Secretion and Cell Viability Using an Epithelial Ovarian Cancer Cell Line

The HTRFR IL-6 assay provides an easy-to-use, sensitive method for the assessment of cytokine secretion from cancer cell models. When run using the 2-plate protocol, the assay can be multiplexed with the Nuclear-ID™ Blue/Red cell viability assay for rapid evaluation of live and dead cell populations. In this application note it is demonstrated that the combined capabilities of the Cytation™3 afford the ability to easily perform each assay with one instrument. The advanced optics and Gen5™ Data Analysis Software features ensure accurate detection of both reader-based and microscopy assays, and provide efficient performance of the entire experimental workflow.