Multiplexed CRISPR-based genome engineering for heterologous protein optimization in E. coli

Traditionally, heterologous proteins are expressed and engineered on plasmids; however, working in plasmid-based systems has drawbacks, such as instability, use of expensive antibiotics for plasmid maintenance, and genetic drift. This application note describes how the Onyx™ workflow can be used to rapidly generate new protein functions in E. coli, with the heterologous gene integrated into the genome.