Multiplexing Secreted Protein Screening, Proliferation and Immunophenotyping of PBMCs on the iQue Screener

T-lymphocyte (T-cell) biology is highly complex and critical to our health and well-being. The iQue® is ideally suited to high content multiparametric screening assays such as the simultaneous measurement of T-cell activation. In this application note, three distinct assays – prolif­eration, cytokine secretion, and IPT – were run together and analyzed in a single read with no washing step.