Multiscale Analysis of Bacteria Population in Legume Root Nodules with "Shuttle & Find"

Correlative light and electron microscopy (CLEM) combines the overview information of fluorescence light microscopy (FLM) with structural details in scanning electron microscope (SEM) for high-content imaging. Hence, CLEM is very useful for investigating infection and colonization of the legume hosts by their rhizobial symbionts. This white paper illustrates a methodology involving the shuttle & find interface of CLEM to enable fast and reliable analysis of the bacterial infection of legume root nodule cells.