New Tools for Drug Discovery: Multiplexing the Monitoring of Ca2+ and cAMP Signaling in Primary Cells Using the FDSS®/µCELL

This application note demonstrates the suitability of primary cells for signaling assays in compound screenings using primary and stem cells expressing luminescent biosensor proteins specific for Ca²⁺ and cAMP. These assay formats are used on the FDSS®/µCELL plate reader to generate pharmacologically relevant data for GPCR ligands.