No-wash, No-lyse Detection of Phagocytic Cells via a pHrodo BioParticles Functional Assay in Human Whole Blood on the Attune NxT Flow Cytometer

Analysis of biological samples in the most physiologically relevant state with minimal sample preparation and manipulation is a key objective in many cell biology workflows. Normally, whole blood samples require time-consuming enrichment and manipulation in preparation for analysis on conventional hydrodynamic focusing flow cytometers. These manipulations can result in loss of rare cell types and undesirable phenotypic changes. This application note outlines a strategy for a no-wash, no-lyse approach to a functional assay using Molecular Probes™ pHrodo™ BioParticles™ Conjugates and the Applied Biosystems™ Attune™ NxT Flow Cytometer to assess phagocyte function in human whole blood.