Non-Destructive Quantification of Cytotoxicity in Live HeLa Cells

Cytotoxicity assays are a crucial step in screening for and developing therapeutic anti-cancer drugs. Most assays designed to measure cytotoxicity in vitro evaluate cell membrane integrity or metabolic activity after exposure, but are typically based on studying a single time point and require disturbing the growth of cells in culture. This application note demonstrates an automated, non-destructive method to monitor and quantify cytotoxicity based on its effects on confluency.