Online Analysis of 25-OH-Vitamin D2/D3 in Plasma and Serum with the EVOQ LC-Triple Quad

This study demonstrates a robust and reliable research method to quantitate 25-OH-vitamin D2 and D3 in plasma and serum samples using the Bruker Advance™ UHPLC with OLE coupled to the EVOQ Elite™ triple quad. The integrated online extraction together with a ClinMass® LC-MS/MS complete kit allows fast and easy sample cleanup. The analyses, with a run time of only 3 minutes, showed excellent linearity of calibration and accuracy as well as a very good intraday and interday precision.