Online Analysis of Immunosuppressants in Whole Blood with the EVOQTM Tandem Quadrupole Mass Spectrometer

Immunosuppressants are used after organ transplantation to prevent allograft rejection by inhibition of the body’s immune system. To improve the scientific understanding of dosage requirements it is necessary to continue research on these compounds. This application note demonstrates a robust, rapid and reliable research method to quantitate the immunosuppressants cyclosporine A, tacrolimus, sirolimus and everolimus in whole blood samples using the Bruker Advance™ UHPLC with OLE (online extraction) coupled to the EVOQ EliteTM tandem quadrupole MS.