Online SPE/LC-MS Method for the Rapid Analysis of Thyroid Hormones in Plasma

Thyroid hormones play critical roles in the regulation of biological processes such as growth and brain development. For the clinical laboratory, measurements of total T4 and total T3, along with estimates of free T4 and free T3 are important for the diagnosis and monitoring of thyroid diseases. An online SPE/LC-MS method was developed for the determination of the thyroid hormones T4, T3, and rT3 from plasma using Ascentis® Express RP-Amide cartridge to trap the analytes, and an Ascentis® Express Phenyl-Hexyl column to resolve them.