Optical Design of CFX96™ Real-Time PCR Detection System

In contrast to the optical design of most real-time PCR systems, the CFX96 and CFX384™ real-time PCR detection systems utilize solid-state optical technology with long-lasting light-emitting diodes for fluorescence excitation and photodiodes for detection. Therefore eliminating the need to sacrifice data collection in one of the channels to normalize to a passive reference. In this study, evidence is presented to support the methodology and show the impact of reaction volume of fluorescence chemistry on replicate samples.