Optical Imaging on the IVIS® SpectrumCT System: General and Technical Considerations for 2D and 3D Imaging

This application note aims to provide the users a general, comprehensive understanding of the operational principles and the underlying differences between BLI and FLI; and to explain and demonstrate technical considerations and animal preparations that can assure optimal 2D/3D optical imaging using the IVIS^® SpectrumCT system. The IVIS^® SpectrumCT is a powerful and versatile optical imaging platform for small animal imaging in pre-clinical settings. Its advanced optical components, including an ultra-sensitive CCD camera and two full range excitation/emission filter sets, enable the system to operate in a broad spectral range, extending from the visible to near infrared (NIR) light.