Optimization of a gradient profile on reversed phase separations of monoclonal antibodies

The analysis of intact monoclonal antibodies by reversed phase HPLC is a common technique for assessing protein variation, such as clipping and heterogeneity due to post-translational modifications. In this technical note, Phenomenex demonstrates how utilizing a bioZen™ 2.6 µm WidePore C4 LC column allows for better resolution and reproducibility through a wider pore core shell particle combined with bioinert hardware. In order to optimize the method, the gradient profile is analyzed.