Optimization of a Multi-Mode Detection Model for Measuring Real-time Cellular Respiration and Mitochondrial Function using Fluorophoric Biosensors

In this application note, new tools designed to optimize cellular metabolism characterization are discussed. One of these tools is a simple mix-and-measure assay compatible with a variety of cellular matrices that utilizes fluorophoric probes to measure oxygen consumption rates (OCR), extracellular acidification (ECA), and intracellular oxygen levels which are useful to inform on the activity of the electron transport chain (ETC) and glycolytic flux. These probes can be detected using standard fluorescence, time-resolved fluorescence, or lifetime fluorescence with reduced background and increased signal dynamic range dependent on the detection mode.