Optimization of CHO Transient Gene Expression (TGE) for Multi-Gram Level Antibody Production: Effects of Expression Construct, Post Transfection Cell Density and Feed Conditions

A transient CHO system that easily scales from milligram production to multi-gram quantities would enable the use of CHO transient gene expression (TGE) as a tool at later stages within the biotherapeutic development pipeline. This poster presents data for the development and fine tuning of several CHO-based antibody production systems that maximize antibody titers and laboratory productivity. The effects of multi- vs. tandem antibody expression plasmids, post transfection cell density, media and feeding conditions will be discussed.