Optimization of Monoclonal Antibody Production Employing SENSOLUXR Non-invasive Sensor Technology

In this application note the effects of supplementary feeding strategies are monitored in real time to develop robust cultivation and production procedures in single use shaker flasks. Novel non-invasive fluorescent probes allow the continuous detection of the pH value and dissolved oxygen saturation during cultivation of animal cells even in lab scale culture formats. This new non-invasive technology offers the opportunity to follow the effects of nutritional state and it allows the identification of starvation conditions.