Optimized Wavelength Scanning Fluorescent Proteins with the SpectraMax Paradigm Platform and TUNE Technology

The SpectraMax® Paradigm® Multi-Mode Detection Platform with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. In this application note it is shown that the TUNE Spectral Optimization Wizard reduces the time required for users to optimize wavelengths for new fluorophores by at least 50% by automatically identifying an optimum wavelength for any given fluorophore. TUNE-optimized wavelengths offer the greatest benefit for assays that use fluorophores with a narrow Stokes’ shift and it is demonstrated that it can result in an 80-fold increase in sensitivity for EGFP detection.