Optimizing analysis and purification of a synthetic peptide using PLRP-S columns

Reversed-phase ion-pair chromatography has become increasingly useful for the analysis and purification of synthetic peptides using mobile phases containing trifluoroacetic acid (TFA) as an ion-pair reagent. This application note from Agilent describes the analysis of a synthetic peptide and how to scale-up methods using an Agilent PLRP-S analytical HPLC column.