Optimizing Chemiluminescent Western Blots

The Odyssey Fc Imaging System is a CCD-based imager that detects chemiluminescent signal, visible signal at 600 nm, and near-infrared fluorescent signals at 700 nm and 800 nm wavelengths. This technical note highlights both chemiluminescent and near-infrared Western blots using this system. It can detect near-infrared fluorescent markers on a chemiluminescent blot, providing a less expensive alternative to HRP-labeled markers.