Optimizing The Picogreen® Assay on BMG LABTECH’s Clariostar Multimode Microplate Reader Using LVF Monochromators

The quantification of nucleic acids samples is a major need in any genetic and molecular biology laboratory. Next to well-known UV absorbance assays (e.g. 260/280 nm), a lot of different fluorescence intensity based DNA quantifi cation assays are offered. In this application note we present DNA quantifi cation data obtained on the CLARIOstar® using the low volume LVis Plate (Fig. 1), as well as 96- and 384-well microplates. For dsDNA Quanit-iT™ PicoGreen® measurements, the CLARIOstar® used either optimized filters or BMG LABTECH’s new LVF Monochromators TM.