Optimizing the Purification of a Standard Chiral Compound Utilizing a Benchtop, Multi-Purpose, Semi-Preparative to Preparative HPLC System

The purpose of this study by Gilson, inc. was to optimize the chiral separation of a standard compound using a stand alone, benchtop, preparative chromatography system. Specifically one that is capable of performing separation by either normal phase or reverse phase chromatography with flow rates up to 100 mL/min. Percent recovery, loading capacity, fraction collection parameters, precision, and its percent impurity are reported for trans-Stilbene oxide using normal phase chromatography.