ORAC Assay for the Determination of Antioxidant Capacity in Foods using the Infinite® 200 PRO Multimode Reader

Reactive oxygen species (ROS) are generated as natural by-products of the cellular metabolism. They are involved in various biological processes, functioning as important signal mediators. However, excess intracellular levels of ROS may result in cell and tissue damage, and are associated with degenerative diseases, most notably cancer. A well-established and reliable method to determine the antioxidant capacity of a substance is the oxygen radical absorbance capacity (ORAC) assay. This application note describes the use of the Infinite® F200 PRO in combination with a commercially available ORAC assay kit, using different beverages as antioxidant samples.