PCR-RFLP Directly from Blood Using Thermo Scientific Phusion Blood Direct PCR Kit

This application protocol describes how PCR-RFLP (restriction fragment length polymorphism) assays can be performed from blood samples without DNA purification. The approach discussed allows for amplification of DNA directly from various starting materials such as blood, mouse ear and tail tissues, plants, and FFPE tissue samples; allowing for genotyping without DNA purification before PCR or digestion.