Peak Shape Improvement Using the Auto-Pretreatment Function of i-Series Plus Integrated Liquid Chromatograph

Ultra-high performance liquid chromatography (UHPLC) requires a system with even lower dispersion than that of conventional high performance liquid chromatography (HPLC). In addition, the UHPLC system commonly employs tubing with an inner diameter of 0.1 mm or less. This is because the internal diameter of columns used for UHPLC systems is small with 2.0 to 3.0 mm being the most popular and therefore more prone to be affected by extra-column dispersion compared to HPLC.