Peptide Cleavage and Protected Cleavage Procedures

In Fmoc synthesis, the removal of the peptide from the solid support is typically accomplished with trifluoroacetic acid (TFA). Because the side chain protecting groups used in Fmoc synthesisare acid labile, a single step both cleaves the peptide from the resin and removes the protecting groups. Various scavenger molecules are added to the TFA to prevent the cleaved protecting groups from reattaching to the peptide. The particular scavengers used depend on the specific peptide sequence.