Performance Characteristics and Assay Procedures for Protein A PhyTip Columns

The PhyNexus PhyTip® columns from PhyNexus enable the capture, purification and enrichment of proteins from a variety of sources. Key to the success of these purification tools is the design of the mechanism to retain the affinity resin bed, with minimum dead volume and maximum capture potential. In this application note a method is presented for the purification of IgG from various sources. It is demonstrated that PhyTip columns have extremely high binding capacity and can bind greater than 100 μg IgG, and can also efficiently recover as little as 200 ng of IgG.