Performance characteristics of the Agilent 1260 Infinity II Hybrid Multisampler

A typical sample introduction principle used for HPLC autosampler modules is the flow-through injection. With this injection mode, the metering device, the loop, and the needle are switched to bypass while drawing the sample. During the injection step, the complete hydraulic path is switched into the flow path and the sample is injected towards the column. In this application note from Agilent Technologies, explore how, in contrast, for Agilent Feed Injection, the sampling part of the autosampler is independent and the pump flow is always connected to the column without any change in the internal volume.