Performance of ACQUITY UPLC M-Class in Proteomics Nanoscale Applications

This application note highlights the performance characteristics of the ACQUITY UPLC M-Class System operating at nanoscale flow rates, illustrating its utility in analyzing complex, tryptic-peptide mixtures. Retention-time reproducibility, with standard deviations of less than 2 seconds, is achieved with peak widths for most peptides of 3.6 seconds FWHM over a short gradient. For analyses of complex mixtures, the system coupled to a SYNAPT G2-Si Mass Spectrometer operating in HDMSE mode has been shown to achieve excellent protein identification rates.