Phenotypic In Vitro Assessment of Compound Effects on Hepatotoxicity Using a Novel Cell Model

Development of predictive in vitro assays for early toxicity evaluation is extremely important for improving the drug development process and reducing drug attrition rates during clinical trials. A promising assay for assessing liver toxicity was developed with Hepregen’s HepatoPac® in vitro liver model. HepatoPac is a 96-well microplate with micro-patterned primary human hepatocytes plus supportive fibroblasts that mimics the physiological microenvironment of the liver. In this study, the hepatocyte islands were imaged with an ImageXpress® Micro XLS high-content imaging system.