Phosphorylation Site Specific Aptamers for Cancer Biomarker Peptide Enrichment and Detection in Mass Spectrometry Based Proteomics

The development of renewable capture reagents that could be produced more efficiently and cost-effectively than monoclonal antibodies is a key topic in diagnostics and therapeutics. This application note shows the development of high-affinity aptamers against specific phosphorylated and unmodified peptide target sequences of certain cancer biomarkers of interest, utilizing a patented, multiplexed “Systematic Evolution of Ligands by Exponential enrichment” (SELEX) approach.