Plasmid DNA transformation in E.coli

As the demand for biotechnological products surges, efficient methods for manipulating microorganisms to produce desired compounds become increasingly important. IKA discusses the crucial role of plasmid DNA transformation in biotechnological research, particularly focusing on its application in Escherichia coli (E. coli) for the production of therapeutic proteins. IKA also highlights the heat shock method as a common technique for introducing exogenous DNA into bacterial cells and outlines the protocol for plasmid DNA transformation. It emphasizes the significance of reliable laboratory equipment, such as pipettes, water baths, thermoshakers, and incubators, in ensuring the success and reproducibility of the transformation process.