Polar Body Biopsy – Advantages of the Eppendorf Micromanipulation System

A well-established technique which is used in preimplantation genetic diagnostics is polar body biopsy, followed by detection of certain chromosomes using fluorescence in-situ hybridization or detection of all chromosomes by comparative genomic hybridization.This application note describes a method for the fast and sensitive handling of the oocyte and polar bodies, using the Eppendorf micromanipulation system with manual injectors and the electronic TransferMan® 4m micromanipulators in combination with the OCTAX Laser Shot™ System.