Postcolumn Reactions Enhance Detection Sensitivity and Selectivity in HPLC Analyses

HPLC analyses of barbiturates can be done rapidly with little sample preparation. Detection, however, presents a set of conflicts, leading to increased likelihood of interference from other sample components. The application note demonstrates a procedure to form a molecular species from an analyte for which the detector has higher sensitivity, or to enable the use of more selective conditions (e.g., a different UV wavelength or fluorescence).