Precipitation of Proteins and Lipid Extraction From Homogenized Rat Skin Tissue

In this study, protein precipitation and bioactive lipid extraction from rat skin tissues is reported. The combination Solid Phase Extraction and liquid chromatography-tandem mass spectrometry (LCMS/MS) approach was used to measure concentrations of lipid mediators in these tissues. Rat skin tissues were homogenized using CKMix50_7mL Precellys^® Lysing Kit and Precellys^® Evolution tissue homogenizer combined with Cryolys^® cooling unit. In addition, temperatures of homogenates were measured to investigate if temperature of complete homogenate of rat skin tissues remained below 40°C.