Principles and solutions of size exclusion chromatography

Size exclusion chromatography (SEC) is a highly versatile separation technique. It is well suited for biomolecules sensitive to changes in pH, concentration of metal ions or cofactors, or harsh environmental conditions. Separations can be performed in the presence of essential ions, cofactors, detergents, urea, or guanidine hydrochloride at high or low ionic strength. Furthermore, SEC can be performed within a wide temperature range covering the requirements of most experiments.

Explore the use of SEC for the purification and separation of biomolecules. Discover how you can optimize results focusing on SEC resins and find common applications and theoretical principles behind this technique.