Probing the human Alzheimer's cortical section using spatial multiplexing

A critical challenge of Alzheimer’s disease (AD) pertains to understanding the spatial microenvironment of AD brain tissue, specifically mapping the different neuronal cell types and expression markers associated with the disease. This type of spatial profiling is crucial for uncovering cellular relationships that could provide insights into disease mechanisms and potential therapeutic strategies. This application note from Leica Microsystems sets out to address these challenges and enhance understanding of AD by utilizing the Cell DIVE multiplexed imaging and AI-powered spatial analysis workflow. This workflow, combining cell-type specific markers, multiplexed tissue imaging, and AI-guided analysis will provide researchers with a deeper understanding of the spatial heterogeneity in the AD brain, paving the way for novel insights.