Production of U-[2H], Thr-g2[¹³CH3] Labeled Proteins for Methyl-TROSY NMR

Isotope labelling has revolutionized the use of biomolecular NMR spectroscopy, allowing the exploration of molecular interactions with high sensitivity and resolution. Introducing labelled Thr, a protein often found at molecular interfaces and involved in binding nucleic acids, would allow a deeper understanding of the interactions at these surfaces. This application note describes a simple biosynthetic strategy that labels these proteins for use in NMR spectroscopy.